Bosch Institute Advanced Microscopy Facility Workshops Summer/Autumn 2016

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1 Bosch Institute Advanced Microscopy Facility Workshops Summer/Autumn 2016 Presented by Dr Louise Cole and Dr Cathy Payne, Advanced Microscopy Facility, Bosch Institute, School of Medical Sciences, The University of Sydney.

2 Microscope Training If you are interested in learning how to use light and fluorescence microscopes, the Bosch Institute Advanced Microscopy Facility (AMF) can offer expert training and support on a range of light and fluorescence microscopes and other specialized imaging methods, including image analysis and processing methods. Summary of Summer/Autumn Workshops 2016 ZEISS AxioScan Slide-Scanning Microscope ZEISS transmitted light, fluorescence, ApoTome.2 and deconvolution Microscope Nuance multi-spectral imaging system Olympus MBF/Stereo Investigator TL Microscope Leica SPEII confocal Microscope ZEISS LSM 510 Meta confocal Microscope PALM Microbeam DuoFlex Combi Laser Microdissection Microscope Light-sheet/Ultramicroscope microscope Cannot find the training workshop you need? For a full list of microscopes, their specifications and related software available at the Bosch AMF please go to: sydney.edu.au/medicine/bosch/facilities/ advanced-microscopy. Training can be offered on one-on-one basis by arrangement with the Microscopy Officer. Cost: In most cases, training workshops are FREE to current registered users of the Bosch Institute Advanced Microscopy Facility. How to register as a user at the Bosch Institute Advanced Microscopy Facility? Current registration forms can be downloaded from the Bosch AMF website: sydney.edu.au/medicine/bosch/facilities/advanced-microscopy/user-support. Fees are listed on this form. If you require training and need to use a number of microscopes at the Bosch AMF, then registering to the AMF is costeffective. Since registration costs cover all access to microscopes and related software and both training and workshops fees. No additional hourly usage fees are charged. Please return completed forms to the Microscopy Officer. All AMF users must be trained by AMF staff on the equipment prior to use. Individual training and workshop fees for non-registered AMF users can be provided on request from the Microscopy Officer.

3 ZEISS AxioScan slide-scanning microscope This microscope has both bright-field and fluorescence imaging capabilities and can create virtual images of whole slides or defined regions of interest. It can hold up to 100 slides at one time and allows high through-put slide-scanning so that you can scan your slides overnight! In this workshop you will learn how to use this microscope independently. Tues Mar 8 th or Weds Mar 9 th am pm. Rm N127, Anderson Stuart Building, F13. Maximum attendees per session, 4. Zeiss transmitted light, fluorescence, ApoTome.2 and deconvolution microscopy workshop This microscope is our most popular wide-field fluorescence microscope. In this workshop you will learn how to set-up for transmitted light, wide-field fluorescence and deconvolution microscopy. Includes ZEN Blue software training. You will also learn how to use the ApoTome.2 for contrast enhancement. Mon Mar 14 th 2.00 pm pm or Thurs Mar 17 th 2.30 pm pm. Rm S443, Anderson Stuart Building, F13. Maximum attendees per session, 5. Nuance multi-spectral imaging system This multi-spectral imaging technology can acquire images at many wavelengths and can therefore better determine fluorophore or stain distribution. It can be used to separate spatially overlapping chromogens and remove autofluorescence from tissues. This microscope has both bright-field and fluorescence imaging capabilities. Mon Mar 21 st 2.00 pm pm. Rm E401, Anderson Stuart Building, F13. Max. attendees per session, 5. Olympus MBF/Stereo Investigator TL Microscope This microscope has a motorised xyz-stage and MBF Stereo Investigator software for design based Stereology, Image Analysis, and 2D Anatomical Mapping. In this workshop, you will learn how to use the Virtual Slice module that automatically creates a montage by collecting a series of contiguous images from a tissue specimen. This software aligns, stitches and blends images into a seamless montage. This microscope is quick and easy to use, is useful for routine bright-field imaging and enables fast navigation to any region of the slide. Thurs Mar 24 th 2.30 pm pm. Rm E401, Anderson Stuart Building, F13. Max. attendees per session, 5.

4 Leica SPEII confocal microscope workshop Upright microscope suitable for imaging fixed and slidemounted specimens. Short workshop on confocal imaging includes multi-colour and z-stack image acquisition. This workshop is popular with beginners to confocal microscopy. Tues Apr 5 th, Weds Apr 6 th or Thurs Apr 7 th am noon. Rm S443, Anderson Stuart Building, F13. Max. attendees per session, 5. ZEISS LSM 510 Meta confocal microscope workshop Short workshop on confocal imaging includes single-, multitrack and z-stack image acquisition. Inverted microscope supports imaging of slide-mounted specimens and single or multi-well culture dishes. This course and microscope is recommended for users with some previous knowledge of fluorescence and/or confocal microscopy. Tues Apr 12 th or Weds Apr 13 th am noon. Rm S443, Anderson Stuart Building, F13. Max. attendees per session, 5. PALM Microbeam DuoFlex Combi Laser Microdissection Microscope workshop Do you want to combine your microscopy with molecular biology tools? This workshop shows you how to laser microdissect selected areas of cells or tissue samples. Dissected samples are catapulted against gravity into collection caps, are contamination-free and ready for further downstream molecular analyses of RNA, DNA and/or protein. Tues Apr 26 th or Weds Apr 27 th am noon. Rm N135, Anderson Stuart Building, F13. Max. attendees per session, 6. Light-sheet microscopy workshop Learn how to use the LaVision BioTec Ultra-microscope II. This microscope generates six focused light sheets to excite samples from the side only and thereby reduce bleaching and photo-toxicity. The emitted fluorescence light is detected perpendicular to the illumination plane. Moving the sample through the light-sheet generates a 3D image stack. The open set-up allows the analysis of cleared samples in any clearing solution or in vivo data acquisition of samples in aqueous media. Perfect for imaging larger cleared samples quickly. Tues May 3 rd or Weds May 4 th am noon. Rm S448, Anderson Stuart Building, F13. Max. attendees per session, 6.

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6 For workshop registration or any further information, please contact: Dr. Louise Cole Tel. No Dr. Cathy Payne Tel. No CRICOS 0026A