10:30 10:40: Introduction, Chris Guérin, Sebastian Munck, Saskia Lippens and Peter O Toole, co-organizers (seminar room)

Transcription

1 Summer School in Advanced Light Microscopy 2015 Jozef Schell seminar room, Fiers-Schell-Van Montagu (FSVM) building Technology park 927, Ghent Day 1 15 june 10:00 10:30: Registration (lobby) 10:30 10:40: Introduction, Chris Guérin, Sebastian Munck, Saskia Lippens and Peter O Toole, co-organizers 10:40 11:20: Lecture 1 The history, theory and basics of light microscopy: light, basic optics, diffraction, lens design and nomenclature, and Köhler illumination, Chris Guérin, Bio Imaging Core Gent, Ghent University, Belgium 11:20 12:00: Practical 1 Setting up Köhler illumination (small cafeteria) 12:00 12:45: Lunch (lobby) 12:45 13:15: Lecture 2 Contrast and imaging methods, phase contrast (PC) and differential interference contrast (DIC), Sebastian Munck, Light Microscopy and Imaging Network LiMoNe, VIB Bio Imaging Core Leuven and VIB Center for the Biology of Disease, KU Leuven, Belgium 13:15 13:50: Practical 2 Setting up PC (small cafeteria) 13:50 14:30: Lecture 3 Reflected light microscopy, fluorescence, fluorochromes and filters, Bob Asselbergh, VIB Department of Molecular Genetics, University of Antwerp, Belgium 14:30 15:20: Coffee break, Practical 3 Designing a multi-color labeling experiment (lobby/cafeteria) 15:20 16:10: Guest Lecture 1 The practical uses of fluorescence: Immunocytochemistry (ICC), flow cytometery and in situ hybridization, Peter O Toole, Imaging and Cytometry Core Facility, University of York, England 16:10 16:40: Practical 4 Designing your own ICC experiment (lobby/cafeteria) 16:40 17:30: Guest Lecture 2 Fluorescence Nanoscopy, Mike Heilemann, EMBL 19:30 Amadeus (sponsored by )

2 Day 2 16 June 09:00 09:50: Guest Lecture 3- Introduction to confocal microscopy, Peter O Toole, Imaging and Cytometry Laboratory, University of York, United Kingdom 09:50 10:10: Lecture 4 Special techniques, TIRF, Evelien Van Hamme Microscopy Core Facility, VIB Department for Molecular Biomedical Research and VIB Bio Imaging Core Gent, Ghent University, Belgium 10:10 10:30: Coffee break (lobby) 10:30 11:15: Practical 5 Looking and seeing in the microscope-part 1 (small cafeteria) 11:15 12:00: Lecture 5 The basics of live cell imaging, Stefan Vinckier, Histology & Imaging Facility, VIB Vesalius Research Center, K.U.Leuven, Belgium 12:00 12:45: Sandwich lunch (lobby) 12:45 13:45: Practical 6 Looking and seeing in the microscope-part 2 (small cafeteria) 13:45 14:45: Guest Lecture 4 Imaging Infection, advances in live cell and tissue imaging and high content microscopy. Spenser Shorte, IMAGOPOLE, Institut Pasteur, Paris, France 14:45 15:00: Coffee break (lobby) 15:00 15:20: Lecture 6 Intro to electron microscopy, Chris Guérin, Microscopy Core Facility, VIB Department for Molecular Biomedical Research and VIB Bio Imaging Core Gent, Ghent University, Belgium 15:20 16:00: Guest Lecture 5 Advanced EM: ultrastructure in 3D. Andreas Schertel, Carl Zeiss, Oberkochen, Germany 16:00 16:15: Short break 16:15 17:00 Guest Lecture 6 Fluorescence confocal imaging of plants, Daniel Van Damme, Plant Systems Biology, VIB-UGent 19:00 21:30: Pakhuis (sponsored by )

3 Day 3 17 June 09:10 09:50: Lecture 7 Miscellaneous microscopy: Fixation, non-adherent cells, tissue slices and whole embryos, light sheet microscopy, microscope maintenance, Chris Guérin, Bio Imaging Core Gent, Ghent University, Belgium 09:50 10:10: Coffee break (atrium) 10:10 11:25: Practical 7 Confocal microscopy: Rotation 1 (see additional schedule) 11:25-12:40 Practical 8 Confocal microscopy: Rotation 2 (see additional schedule) 12:40 13:20: Sandwich lunch (atrium) 13:20 14:35: Practical 9 Confocal microscopy: Rotation 3 (see additional schedule) 14:35 15:00 Coffee break (atrium) 15:00 16:15: Practical 10 Confocal microscopy: Rotation 4 (see additional schedule) 16:15-16:40 Break 16:40-17:30 Guest Lecture 7 - Correlative light and electron microscopy (CLEM), Lucy Collinson, Electron Microscopy Unit, London Research Institute (W122) Evening free to enjoy Ghent

4 Day 4 18 June 09:00 09:40: Lecture 8: Advanced confocal techniques, FRAP, FLIM, FRET, Eef Parthoens, Bio Imaging Core Gent, Ghent University, Belgium 10:30 10:45: Coffee break (atrium) 10:45 12:00: Practical 11 Confocal microscopy: Rotation 5 (see additional schedule) 12:00 13:00: Sandwich lunch (atrium) 13:00 14:15: Practical 12 Confocal microscopy: Rotation 6 (see additional schedule) 14:15 14:45 Coffee break (atrium) 14:45 16:00 Practical 13 Confocal microscopy: Rotation 7 (see additional schedule) 16:00 17:15 Practical 14 Confocal microscopy: Rotation 8 (see additional schedule) 17:20-18:00 Lecture 9: Fluorescent probes and delivery strategies. Sebastian Munck, Light Microscopy and Imaging Network LiMoNe, VIB Bio Imaging Core Leuven and VIB Center for the Biology of Disease, KU Leuven, Belgium (small cafeteria) 18:00 20:00 Beer and Bar-B-Q (DMBR patio) (Sponsored by )

5 Day 5 19 June 09:15 10:05: Lecture 10: Overview on Image processing methods, Benjamin Pavie, Microscopy Core Facility, VIB Department for Molecular Biomedical Research and VIB Bio Imaging Core Gent, Ghent University, Belgium 10:05 10:45: Lecture 11: Advanced image processing Jean-Yves Tinevez, IMAGOPOLE, Institut Pasteur, Paris, France 10:45 11:15 Coffee break (atrium) 11:15 12:05: Practical 15 Image processing (Interactive) Examples of operations using Image J and Fiji, Eef Parthoens, Benjamin Pavie & Jean-Yves Tinevez, Microscopy Core Facility, VIB Department for Molecular Biomedical Research and VIB Bio Imaging Core Gent, Ghent University, Belgium and IMAGOPOLE, Institut Pasteur, Paris, France (small cafeteria) 12:05 12:45 Sandwich lunch (atrium) 12:45 15:00 Practical 15 continued 15:00 Course ends