Image Viewing. with WebScope
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1 Image Viewing with WebScope
2 WebScope Overview WebScope A platform independent digital slide viewer. Does not require client side software installation. Can be used from any workstation (Macintosh, Linux, etc.). Uses any Internet browser that supports Adobe Flash Player. Certain WebScope features are only available when used with an Aperio system in which digital slides are served by Aperio ImageServer. User permission are controlled by the role assigned to the user. Page 2
3 WebScope Features WebScope Features Include: Create and view slide annotations. Analyze digital slides. Adjust brightness and contrast. Navigate and zoom the digital slide. Annotation tools Annotation window Page 3
4 WebScope Tools WebScope Main Display Main toolbar Thumbnail Navigation toolbar Page 4
5 WebScope Tools Toolbar Summary Integrated Color Management Show/Hide Thumbnail Adjust Brightness /Contrast Save Annotations Negative Pen Ellipse Image Information WebScope Information Show/Hide Navigation Toolbar Analyze Digital Slide Pan Pen Ruler Rectangle Arrow WebScope Quick Reference Show/Hide Annotations Window Page 5
6 WebScope Tools Navigation Toolbar Summary Current Magnification Level Move Indicated Direction Home: fits entire digital slide to fit window. Magnification Slider bar Zoom In/Out Create Link to Digital Slide Page 6
7 WebScope Basic Features Open Slide in WebScope Depending on the Aperio product, the method of opening a digital slide in WebScope differs. Spectrum and Spectrum Education: Click the thumbnail image to launch WebScope. If ImageScope is installed, hold down the W key while you click the thumbnail image. Page 7
8 WebScope Basic Features Open Slide in WebScope KickStart for Educators: Access the website served by KickStart. Click WebScope link under the digital slide to be opened. Page 8
9 WebScope Digital Slide Navigation Navigation Panning - use the mouse to left-click and drag the image (this is the fastest method). Use the arrows to move in one direction. Click anywhere in the thumbnail to move to a new location quickly. The thumbnail image shows the entire digital slide. Page 9
10 WebScope Digital Slide Navigation Change Magnification Quickly zoom to max magnification by double-clicking on the image. Set the magnification to a specific level using the slider icon. Zoom in and out using the icons. Rest the view to fit the entire image in the main window using the icon. Page 10
11 WebScope Annotations Annotations Window Displays details about annotations that have been drawn on the digital slide. Use the window to: Show or hide annotations on main window. Place text labels on the annotations. Click the icon to show or View analysis results. Change the color of the drawing. hide the annotations window. Layers of annotations display in different colors. Layer Region displays data for each region within the Layer. Page 11
12 WebScope Annotations Annotation Drawing Tools Annotations can define a region of interest and measure an object on the digital slide. To use a tool, select tool, left-click and move across the image. Pen * Negative Pen*(excludes area) Ruler (measures in microns) Rectangle* Ellipse Arrow *These annotation tools can be used to define regions to perform or exclude Image Analysis. Tip: Right click to select an annotation tool. Page 12
13 WebScope Annotations Layers and Attributes Each group of annotations can exist on a separate layer. Organize information by pathologist or department. Each layer is given a separate color to easily differentiate one from another. Each layer can have one or more attributes. Attributes can be text stored with the layer or analysis data. Show/hide layers Change Color Add Layer Delete Selected Layer Delete All Layers Layer Attributes displays combined image analysis results for all regions. Page 13
14 WebScope Image Analysis Analyze a Digital Slide in WebScope Use the drawing tools to define an area of interest (unless doing whole slide analysis). Click on the main toolbar to open the Algorithm job window. Select a macro, region of analysis and if a markup image is to be generated and click Analyze. A status message informs the job status. Page 14
15 WebScope Image Analysis Analysis Results Quantitative results are available in a new layer in the Annotations window. Page 15
16 WebScope Image Analysis Markup Image If requested, a markup image will be displayed with the digital slide. For information on the meaning of the different pseudo-colors used in the markup, see the user s guide for the particular algorithm you are using. Page 16
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