Laser Capture Microdissection, Methods for Microgenomic Analysis. Location: FAES BioTech One Cloister Ct., Building 60, RM 60, Bethesda MD, 20814

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1 Agenda Laser Capture Microdissection, s for Microgenomic Analysis Location: FAES BioTech One Cloister Ct., Building 60, RM 60, Bethesda MD, Monday Welcome and Introductions Introduction to Laser Microdissection, the Course and the Technology : Jeffrey Hanson, NIH/NCI Day 1 Tissue Specimens, Preservation and Storing 1 : Virginia Espina, Ph.D., George Mason Univ. Day 1 Pathology and Microdissected Samples 11:10am - 12:10pm (Avi Rosenburg, MD, Ph.D., NIH/NIDDK, Children's National Medical Center) Day 1 Lunch 12:10pm - 1:00 pm Afternoon Lab: Slide Preparation and Introduction to Microdissection Systems 1:00 to 6:00. Students divided in four groups (A,B,C, and D) These groups will be for the whole week 1:15pm : Dr. Kulesh and Dr. Kalina Day 1 1:00-2:00 2:00-3:00 3:00-4:00 4:00-5:00 5:00-6:00 Slide Preparation A None D C B Leica B A None D C Arcturus XT C B A None D Zeiss PALM D C B A None MMI None D C B A 8/5/10 1

2 Tuesday Working with Hazardous Samples 10:15am Microdissection and DNA Methylation, DNA Studies 10:30am 12:00pm : Todd Bell, US Army : Jeffrey Hanson, NIH/NCI Day 2 Day 2 Afternoon Lab: Microdissection of a Slide and DNA Extraction Rotation I - 1:00-3:00 Group A - Leica LMD7000 (Rm. 060) Group B - MDS Arcturus XT (Rm. 134 Group C - Zeiss PALM (Rm. 162) Group D - MMI CellCut (Rm. 134 back) Rotation II - 3:00-5:00 Group A - MMI CellCut (Rm. 134 back) Group B - Leica LMD7000 (Rm. 060) Group C - MDS Arcturus XT (Rm. 134 Group D - Zeiss PALM (Rm. 162) 3:00pm 3:00pm Day 2 : : Day 2 Day 2 8/5/10 2

3 Wednesday Microdissection Project Design 8:30am RNA and qpcr and Microarray from Microdissected Samples Mass Spec and Proteomics with Microdissected Samples 10:05am 11:05pm 11:10am 12:10pm Afternoon Lab: DNA isolation PCR set up, and Rotations on Microdissection Systems 6:00pm : (Yelena Golubeva, Ph.D. Lawrence Sternberg, Ph.D., FNLCR) : Claudius Mueller, Ph.D., George Mason Univ. Virginia Espina, Ph.D., George Mason Univ. Rotation I - 1:00-3:00 Group A - Leica LMD7000 (Rm. 060) Group B - MDS Arcturus XT (Rm. 134 Group C - Zeiss PALM (Rm. 162) Group D - MMI CellCut (Rm. 134 back) 2:45pm : Rotation II - 3:00-5:00 Group A - MMI CellCut (Rm. 134 back) Group B - Leica LMD7000 (Rm. 060) Group C - MDS Arcturus XT (Rm. 134 Group D - Zeiss PALM (Rm. 162) 2:45pm 4:30pm : DNA Isolation and Cleanup (All groups - Rm 60) 4:30-6:00 4:30pm 6:00pm : Jeffrey Hanson Zoe Bogus 8/5/10 3

4 Thursday Proteomics on Microdissected FFPE Samples : Adel Blackler, Ph.D., NantOmics RNA analysis from Microdissected FFPE samples 10:10am 11:10pm : Jeffrey Recent LCM Publications: Pushing the Envelope 11:15am 12:00pm : Jeffrey Afternoon Lab - Hands on Rotations on Microdissection Systems and Demonstrations of Special Techniques. Lab Rotation 1: Hands on Laser Microdissection (1:00-2:00) Lab Rotation 2: Hands on Laser Microdissection (2:00-3:00) Lab Rotation 3: Hands on Laser Microdissection (3:00-4:00) Lab Rotation 4: Hands on Laser Microdissection (4:00-5:00) : 8/5/10 4

5 Friday xmd and Next Generation Sequencing 9:00-10:25 10:30am : (Avi Rosenburg, MD, Ph.D., NIH/NIDDK, Children's National Medical Center) Day 5 All groups, Closure: Discussion of Lab Results 10:35am 11:35am : Jeffrey Day 5 Lunch 11:35am 12:35pm Afternoon Lab Sessions: Sign Up for Rotation: Special Topics and Applications on Instruments 12:35pm 3:35pm : Day 5 Sign Up for Rotation / Special Topics on Instruments. a. Lab Rotation 1: Hands on Laser Microdissection (12:35-1:35) b. Lab Rotation 2: Hands on Laser Microdissection (1:35-2:35) c. Lab Rotation 3: Hands on Laser Microdissection (2:35-3:35) 12:35pm 3:35pm : Day 5 8/5/10 5